1. What Is Reconstitution?

Reconstitution is the process of adding a liquid diluent to a dry substance to prepare a solution of known concentration. This is commonly done with research compounds from Tocris Bioscience and other suppliers.

2. How Does The Calculator Work?

The calculator uses the basic concentration formula:

Where:

• \( C \) — Concentration (mg/mL)
• \( mass \) — Mass of the compound (mg)
• \( volume \) — Volume of solvent (mL)

Explanation: The formula calculates the concentration by dividing the mass of the compound by the volume of solvent used for reconstitution.

3. Importance Of Accurate Reconstitution

Details: Accurate reconstitution is critical for experimental reproducibility. Incorrect concentrations can lead to invalid results or failed experiments.

4. Using The Calculator

Tips: Enter the mass of your compound in mg and the desired volume of solvent in mL. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: What solvent should I use for reconstitution?
A: Always use the solvent recommended by the manufacturer. For Tocris compounds, this is typically DMSO, water, or ethanol.

Q2: How should I store reconstituted solutions?
A: Most reconstituted solutions should be aliquoted and stored at -20°C or lower to prevent degradation.

Q3: Can I use this calculator for other brands?
A: Yes, this calculator works for any reconstitution where you know the mass of compound and volume of solvent.

Q4: What if my compound is in µg rather than mg?
A: Convert µg to mg (divide by 1000) before using the calculator.

Q5: How precise should my measurements be?
A: Use analytical balances for mass and calibrated pipettes for volume to ensure maximum accuracy.