1. What is Reconstitution?

Reconstitution is the process of adding a solvent (typically sterile water or buffer) to a lyophilized (freeze-dried) powder to prepare it for use. Tocris is a leading supplier of bioactive small molecules for research.

2. How Does the Calculator Work?

The calculator uses the basic concentration formula:

Where:

• \( C \) — Concentration (mg/mL)
• \( mass \) — Mass of compound (mg)
• \( volume \) — Volume of solvent (mL)

Explanation: This simple formula calculates the concentration of a solution after reconstitution.

3. Importance of Accurate Reconstitution

Details: Proper reconstitution is critical for experimental success. Incorrect concentrations can lead to inaccurate dosing, failed experiments, or even toxic effects in cell cultures.

4. Using the Calculator

Tips:

• Use the mass indicated on your Tocris product vial
• Measure solvent volumes precisely
• Ensure complete dissolution before use
• Consider making stock solutions for repeated use

5. Frequently Asked Questions (FAQ)

Q1: What solvent should I use for reconstitution?
A: Follow the manufacturer's recommendations. Common solvents include DMSO, ethanol, or sterile water depending on the compound's solubility.

Q2: How should I store reconstituted solutions?
A: Most reconstituted solutions should be aliquoted and stored at -20°C or lower to prevent degradation.

Q3: Can I reuse reconstituted solutions?
A: It depends on the compound's stability. Avoid multiple freeze-thaw cycles by making small aliquots.

Q4: What if my compound doesn't dissolve completely?
A: Try gentle warming or sonication, but check the compound's stability first. Never heat DMSO solutions above room temperature.

Q5: How accurate is this calculator?
A: The calculation is mathematically precise, but actual concentration depends on accurate measurement of mass and volume.