1. What is Reconstitution Calculator?

The Reconstitution Calculator helps researchers determine the concentration of a solution when dissolving a known mass of compound (like Tocris products) in a specific volume of solvent.

2. How Does the Calculator Work?

The calculator uses the basic concentration formula:

Where:

• \( C \) — Concentration (mg/mL)
• \( mass \) — Mass of compound (mg)
• \( volume \) — Volume of solvent (mL)

Explanation: This simple equation relates the amount of solute to the volume of solution, giving the concentration.

3. Importance of Concentration Calculation

Details: Accurate concentration calculation is crucial for preparing solutions with precise concentrations needed for experiments, especially in biochemical and pharmacological research.

4. Using the Calculator

Tips: Enter the mass of your compound in mg and the volume of solvent in mL. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why is reconstitution important in research?
A: Precise reconstitution ensures experimental reproducibility and accuracy in dose-response studies.

Q2: What are common solvents used for reconstitution?
A: DMSO, water, ethanol, or buffer solutions depending on compound solubility.

Q3: Should I account for compound purity?
A: Yes, for highly accurate work, adjust the mass based on the purity percentage of your compound.

Q4: How should I store reconstituted solutions?
A: Follow manufacturer recommendations - often at -20°C in aliquots to avoid freeze-thaw cycles.

Q5: Can I use this for non-Tocris products?
A: Yes, this calculator works for any compound where you know the mass and volume.