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Peptide Reconstitution Formula:
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Peptide reconstitution is the process of dissolving lyophilized (freeze-dried) peptide powder in a solvent (typically bacteriostatic water) to create a solution of known concentration for research or therapeutic use.
The calculator uses the basic concentration formula:
Where:
Explanation: This simple formula calculates the concentration of the peptide solution by dividing the mass of peptide by the volume of solvent used.
Details: Proper reconstitution is critical for ensuring accurate dosing in peptide therapy. Incorrect concentrations can lead to underdosing (reduced efficacy) or overdosing (potential side effects).
Tips: Enter the mass of peptide in milligrams and the volume of solvent in milliliters. Both values must be positive numbers. The calculator will output the concentration in mg/mL.
Q1: What solvent should I use for peptide reconstitution?
A: Bacteriostatic water (0.9% benzyl alcohol) is most common for research purposes. For clinical use, follow specific protocol instructions.
Q2: How should I store reconstituted peptides?
A: Most peptides should be stored at 2-8°C (refrigerated) and used within a few weeks, though stability varies by peptide.
Q3: Can I use sterile water instead of bacteriostatic water?
A: Sterile water can be used but offers no preservative properties. Reconstituted peptides in sterile water should typically be used immediately or within 24 hours.
Q4: How do I calculate dosing from the concentration?
A: Dose (mg) = Concentration (mg/mL) × Volume to inject (mL). Always verify calculations with a healthcare professional.
Q5: Why is my peptide not dissolving completely?
A: Some peptides require gentle swirling or slight acidity (adding a drop of acetic acid). Never vortex as this may damage the peptide structure.