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Reconstitution Formula:
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Peptide reconstitution is the process of dissolving lyophilized (freeze-dried) peptides in a suitable solvent (typically bacteriostatic water) to prepare them for research or therapeutic use. The concentration of the resulting solution depends on the amount of peptide and the volume of diluent used.
The calculator uses the simple concentration formula:
Where:
Explanation: This calculation determines how concentrated your peptide solution will be based on how much diluent you add to a given amount of peptide.
Details: Proper reconstitution is crucial for achieving the desired concentration for experiments or treatments. Incorrect concentrations can lead to inaccurate dosing and potentially invalid experimental results.
Tips: Enter the mass of your peptide in milligrams and the volume of diluent you plan to use in milliliters. Both values must be greater than zero.
Q1: What's the best diluent for peptide reconstitution?
A: Bacteriostatic water (0.9% benzyl alcohol) is most common for research purposes, as it prevents bacterial growth. For clinical use, sterile water or saline may be preferred.
Q2: How should I store reconstituted peptides?
A: Most peptides should be stored at 4°C for short-term use or aliquoted and frozen at -20°C for long-term storage. Some peptides require special handling.
Q3: How long do reconstituted peptides remain stable?
A: Stability varies by peptide, but most remain stable for several weeks when refrigerated. Always check specific stability data for your peptide.
Q4: Can I use this calculator for other compounds?
A: Yes, this calculator works for any substance where you need to determine concentration based on mass and volume.
Q5: What if my peptide doesn't dissolve completely?
A: Some peptides require gentle heating, vortexing, or slight pH adjustment. Consult the peptide's datasheet for specific reconstitution instructions.