1. What is Peptide Reconstitution?

Peptide reconstitution is the process of mixing lyophilized (freeze-dried) peptide powder with bacteriostatic water to create an injectable solution. The concentration of the resulting solution depends on the amount of peptide and the volume of water used.

2. How Does the Calculator Work?

The calculator uses the simple concentration formula:

Where:

• \( C \) — Concentration (mg/mL)
• \( mg \) — Mass of peptide (milligrams)
• \( mL \) — Volume of bacteriostatic water (milliliters)

For dose calculation: \[ \text{Injection Volume (mL)} = \frac{\text{Desired Dose (mcg)} / 1000}{C} \]

3. Importance of Accurate Reconstitution

Details: Proper reconstitution ensures accurate dosing, which is critical for both effectiveness and safety when using peptide therapies. Incorrect concentrations can lead to underdosing or potentially dangerous overdosing.

4. Using the Calculator

Tips:

• Enter the total mass of your peptide in mg
• Enter the volume of bacteriostatic water you'll use in mL
• Optionally enter your desired dose in mcg to calculate the required injection volume
• Always use sterile techniques when reconstituting peptides

5. Frequently Asked Questions (FAQ)

Q1: What type of water should I use for reconstitution?
A: Bacteriostatic water (0.9% benzyl alcohol) is recommended as it inhibits bacterial growth. Sterile water can be used but the solution must be used immediately or refrigerated for very short-term storage.

Q2: How should I store reconstituted peptides?
A: Most peptides should be refrigerated (2-8°C) after reconstitution. Some may require freezing. Always check the specific storage requirements for your peptide.

Q3: How long do reconstituted peptides last?
A: This varies by peptide but typically 1-4 weeks when refrigerated in bacteriostatic water. Some peptides are more stable than others.

Q4: Can I use normal saline instead of bacteriostatic water?
A: Some peptides can be reconstituted with normal saline, but this may affect stability. Always check the specific requirements for your peptide.

Q5: How do I measure very small injection volumes accurately?
A: Use insulin syringes (U-100) which allow precise measurement of small volumes. For very small doses (<0.05mL), consider using higher concentration solutions.