1. What is Peptide Reconstitution?

Peptide reconstitution is the process of dissolving lyophilized (freeze-dried) peptides in a suitable solvent to achieve a desired concentration for experimental or therapeutic use.

2. How Does the Calculator Work?

The calculator uses the basic concentration formula:

Where:

• \( C \) — Concentration (mg/mL)
• \( mg \) — Mass of peptide (mg)
• \( mL \) — Volume of solvent (mL)

Explanation: This simple formula helps researchers prepare peptide solutions at precise concentrations for consistent experimental results.

3. Importance of Accurate Reconstitution

Details: Proper reconstitution is critical for maintaining peptide stability, ensuring correct dosing in experiments, and achieving reproducible results in research.

4. Using the Calculator

Tips: Enter the mass of your peptide in mg and the volume of solvent you plan to use in mL. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: What solvent should I use for peptide reconstitution?
A: Sterile water or bacteriostatic water is commonly used. Some peptides may require specific solvents like acetic acid or DMSO for proper solubility.

Q2: How should I store reconstituted peptides?
A: Most peptides should be aliquoted and stored at -20°C. Avoid repeated freeze-thaw cycles which can degrade the peptide.

Q3: What concentration should I use for my experiment?
A: This depends on your specific protocol. Typical working concentrations range from 0.1-1 mg/mL for most applications.

Q4: How long do reconstituted peptides remain stable?
A: Stability varies by peptide, but most remain stable for weeks to months when properly stored. Always check literature for your specific peptide.

Q5: Can I use this calculator for proteins as well as peptides?
A: Yes, the same formula applies for protein reconstitution, though molecular weight considerations may affect your final working concentration.