1. What is the Reconstitution Calculator?

The R&D Systems Reconstitution Calculator helps researchers determine the concentration of reconstituted proteins or other biological materials by calculating the ratio of mass to volume.

2. How Does the Calculator Work?

The calculator uses the basic concentration formula:

Where:

• \( C \) — Concentration (mg/mL)
• \( mass \) — Mass of the substance (mg)
• \( volume \) — Volume of solvent (mL)

Explanation: This simple formula calculates the concentration of a solution when you know the mass of solute dissolved in a specific volume of solvent.

3. Importance of Proper Reconstitution

Details: Accurate reconstitution is critical for experimental reproducibility. Incorrect concentrations can lead to failed experiments or misleading results.

4. Using the Calculator

Tips: Enter the mass in mg and volume in mL. Both values must be positive numbers. For best results, use precise measurements.

5. Frequently Asked Questions (FAQ)

Q1: What if my protein vial contains µg amounts?
A: Convert µg to mg (divide by 1000) before entering the value, or modify the calculator to work with µg/µL.

Q2: How should I choose the reconstitution volume?
A: Consider your desired final concentration and the solubility of your protein. Check the product datasheet for recommendations.

Q3: Should I account for the lyophilized cake volume?
A: Generally no, as it's negligible compared to the solvent volume. Use the exact solvent volume added.

Q4: How precise should my measurements be?
A: Use the most precise balance and pipettes available. For small volumes, consider using a dilution series.

Q5: Can I use this for non-protein reagents?
A: Yes, this calculator works for any substance where you need to determine concentration from mass and volume.