1. What is Molarity of Antibody?

Molarity (M) is a measure of the concentration of a solution expressed as moles of solute per liter of solution. For antibodies, it represents the number of antibody molecules per unit volume.

2. How Does the Calculator Work?

The calculator uses the molarity formula:

Where:

• \( M \) — Molarity (mol/L)
• \( \text{concentration} \) — Antibody concentration in mg/mL
• \( \text{MW} \) — Molecular weight of the antibody in g/mol

Explanation: The formula converts mass concentration (mg/mL) to molar concentration (mol/L) by dividing by the molecular weight.

3. Importance of Molarity Calculation

Details: Knowing the molar concentration of antibodies is crucial for experimental design, particularly in immunoassays, Western blotting, and other applications where precise antibody concentrations are required.

4. Using the Calculator

Tips: Enter the antibody concentration in mg/mL and the molecular weight in g/mol. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: How do I find the molecular weight of my antibody?
A: The molecular weight can typically be found in the product datasheet. For IgG antibodies, it's approximately 150,000 g/mol.

Q2: Why is molarity important for antibodies?
A: Molarity allows for accurate comparison and dilution of antibodies based on the number of molecules rather than mass, which is more relevant for biological interactions.

Q3: What's a typical antibody concentration range?
A: Stock antibody solutions are often 0.1-10 mg/mL, with working concentrations typically in the nM to µM range.

Q4: Does this calculation work for antibody fragments?
A: Yes, as long as you use the correct molecular weight for the fragment (e.g., ~50,000 g/mol for Fab fragments).

Q5: How do I convert molarity back to mg/mL?
A: Multiply molarity (M) by molecular weight (g/mol) to get concentration in g/L, then divide by 1000 to convert to mg/mL.