1. What is the Reconstitution Formula?

The reconstitution formula \( C = \frac{mass}{volume} \) is used to calculate the concentration of a solution after dissolving a known mass of solute in a specific volume of solvent. This is essential for preparing accurate working solutions from lyophilized proteins or other reagents.

2. How Does the Calculator Work?

The calculator uses the basic reconstitution formula:

Where:

• \( C \) — Concentration (μg/mL)
• \( mass \) — Mass of solute (μg)
• \( volume \) — Volume of solvent (mL)

Explanation: The formula simply divides the mass of the solute by the volume of solvent to determine concentration.

3. Importance of Accurate Reconstitution

Details: Proper reconstitution is critical for experimental reproducibility, accurate dosing in cell culture, and ensuring consistent results in biological assays.

4. Using the Calculator

Tips: Enter mass in micrograms (μg) and volume in milliliters (mL). Both values must be positive numbers. The calculator will compute the concentration in μg/mL.

5. Frequently Asked Questions (FAQ)

Q1: What if my protein comes in μg and I need ng/mL?
A: First calculate μg/mL, then multiply by 1000 to convert to ng/mL (1 μg/mL = 1000 ng/mL).

Q2: How should I choose the reconstitution volume?
A: Consider your desired final concentration and the minimum volume needed to fully dissolve the solute. Check the product datasheet for recommendations.

Q3: Does this account for dilutions?
A: No, this calculates stock concentration only. For working concentrations, you'll need to perform additional dilution calculations.

Q4: What about molecular weight for molar concentrations?
A: This calculator gives mass concentration. For molarity, you'd need to divide by the molecular weight (MW) of the compound.

Q5: How precise should my measurements be?
A: Use analytical balances for mass and calibrated pipettes for volume. Precision depends on your application requirements.